Short Title:DNA Analysis I
Full Title:DNA Analysis I
Language of Instruction:English
Module Code:DNAA H3002
Credits: 5
Field of Study:Science
Module Delivered in 7 programme(s)
Reviewed By:JOHN BEHAN
Module Description:This module has been designed to build on core competencies in DNA extraction and amplification achieved in semester 4. The module will cover the techniques of DNA hybridization (including probe design and labelling), and of DNA sequencing both of which underpin genetic analysis. Following on from the fundamentals of PCR covered in semester 4, this module also covers applications of DNA profiling methodologies to microbial, animal and food samples. Theoretical aspects of this module are complemented by laboratory experiments delivered concurrently.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Demonstrate an understanding of the fundamentals, optimization and applications of PCR Adaptations in prokaryotes and Eukaryotes
LO2 To demonstrate an understanding and to be able to differentiate between PCR and non-PCR based microbial typing techniques
LO3 Apply electrophoretic techniques to the analysis of DNA
LO4 Choose an appropriately labelled probe and perform a hybridisation reaction for the detection of DNA sequences
LO5 Demonstrate the principles of dideoxy/Sanger sequencing and discuss the impact of the human genome project on genetic analysis

Module Content & Assessment

Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Continuous Assessment Continuous Assessment Examination(s) 1,2,3,4,5 30.00 n/a
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Formal Exam End-of-Semester Final Examination 1,2,3,4,5 70.00 End-of-Semester

TU Dublin – Tallaght Campus reserves the right to alter the nature and timings of assessment


Module Workload

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture Course content delivered by lecturer in three 1hour lecture periods 3.00 Every Week 3.00
Independent Learning The student will be required to consolidate the knowledge imparted in lectures through independent learning, the duration of which will depend on the individual learner. 3.00 Every Week 3.00
Total Weekly Learner Workload 6.00
Total Weekly Contact Hours 3.00
This module has no Part Time workload.

Module Resources

Required Book Resources
  • Andreas Hofmann,Samuel Clokie 2018, Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, 8th Ed., Cambridge University Press [ISBN: 9781316614761]
Recommended Book Resources
  • Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays 2010, Principles and Technical Aspects of PCR Amplification [ISBN: 978-9048175796]
Required Article/Paper Resources
  • Various relevant articles will be referenced in the module to ensure that material covered is up-to-date
This module does not have any other resources

Module Delivered in

Programme Code Programme Semester Delivery
TA_SABIO_B Bachelor of Science (Honours) in Bioanalytical Science 6 Mandatory
TA_SPHAR_B Bachelor of Science (Honours) in Pharmaceutical Science 6 Group Elective 2
TA_SADNF_B Bachelor of Science Hons in DNA and Forensic Analysis 6 Mandatory
TA_SBIOL_D Bachelor of Science in Bio Analysis (1 year add-on Bachelor Degree) 6 Mandatory
TA_SASCI_D Bachelor of Science in Bioanalysis or Chemical Analysis 6 Group Elective 1
TA_SADNF_D Bachelor of Science in DNA and Forensic Analysis 6 Mandatory
TA_SAPHR_D Bachelor of Science in Pharmaceutical Science 6 Group Elective 2