Short Title:Downstream Processing
Full Title:Downstream Processing
Language of Instruction:English
Module Code:BIOT H3042
Credits: 10
Field of Study:Pharmacy
Module Delivered in 1 programme(s)
Reviewed By:JOHN BEHAN
Module Description:This module aims to provide the student with the theoretical and practical fundamentals of protein structure, isolation, purification and characterisation and their application to down stream processes in the biopharmaceutical industry.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Explain in detail, the structure and physicochemical properties of proteins and how they are exploitned in downstream purification
LO2 Compare and contrast the different chromatographic methods for protein purification, capture, polishing and viral clearance
LO3 Describe the stages involved in scale-up of chromatography, CIP & SIP, and the qualification of columns.
LO4 Understand the operation and limitations of the protein separation and concentration techniques required for lab scale and industrial processing
LO5 Perform and report on laboratory methods for the purification and concentration of proteins

Module Content & Assessment

Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Practical/Skills Evaluation Perform chromatographic methods for the separation and purification of proteins. Perform methods for the concnetration of protein samples using TFF including intergrity testing of filters. 2,5 25.00 Sem 1 End
Continuous Assessment Essay/assignment to describe the development and scale-up of a workable protein purification protocol 1,2,3,4 25.00 Week 7
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Formal Exam End-of-Semester Final Examination 1,2,3,4 50.00 End-of-Semester

TU Dublin – Tallaght Campus reserves the right to alter the nature and timings of assessment


Module Workload

This module has no Full Time workload.
Workload: Part Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture No Description 3.00 Every Week 3.00
Assignment Essay report to design a workable protein purification protocol 2.00 Every Week 2.00
Laboratories Performa nd report on assigned lab techniqiues 5.00 Every Month 1.25
Total Weekly Learner Workload 6.25
Total Weekly Contact Hours 4.25

Module Resources

Recommended Book Resources
  • Stefan Behme. 2009, Manufacturing of pharmaceutical proteins : from technology to economy, Wiley-VCH Weinheim
  • Gary Walsh. 2007, Pharmaceutical biotechnology : concepts and applications, John Wiley & Sons Chichester, England ; Hoboken, NJ
  • G. Walsh 2001, Protein Biotechnology and Biochemistry, nd ed Ed., Wiley
  • G. Walsh 1998, Biopharmaceuticals: Biochemistry and Biotechnology, Wiley
  • Michael Verrall 1996, Downstream processing of Natural Products – A practical Handbook, Wiley
  • Eugene J. McNally 2000, Protein Formulation and Delivery, Marcel Dekker Inc
This module does not have any article/paper resources
Other Resources
  • Moodle: All lecture notes and x5 eBooks from Chromatography Resin Manufacturers on differnt kinds of protein purification

Module Delivered in

Programme Code Programme Semester Delivery
TA_SPHAR_D Bachelor of Science in Pharmaceutical Technology 6 Group Elective 1