Short Title:Upstream Processing
Full Title:Upstream Processing
Module Code:BIOT H3041
Credits: 10
Field of Study:Pharmacy
Module Delivered in 1 programme(s)
Reviewed By:JOHN BEHAN
Module Description:This module aims to provide the student with the theoretical and practical fundamentals of recombinant DNA technology and the application of this technology to biopharmaceutical production. The module initially focuses on providing an understanding of how proteins are made and how they can be genetically engineered, and on key areas of the immune system. These biological systems are then applied to the upstream processes of biopharmaceutical production.
Learning Outcomes
On successful completion of this module the learner will be able to:
LO1 Explain and detail the role of Biotechnology in the discovery and development of biopharmaceutical products and their manufacture.
LO2 Identify and compare expression systems for the generation of biopharmaceutical products in biomanufacturing cells.
LO3 Describe animal cell culture systems, their development and genetic engineering to express therapeutic proteins and vaccines and the requirements for their industrial scale production.
LO4 Describe the design and process control requirements of fermentors and bioreactors in common use in the industry
LO5 Perform and report on laboratory studies for the isolation and maniplulation of cellular and plasmid DNA.

Module Content & Assessment

Course Work
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Practical/Skills Evaluation Perform and report on the isolation of DNA, minipreparation of plasmid DNA, determination of its purity and concentration by UV spectrophotometry, restriction endonuclease digestion of DNA samples and visualisation of DNA on agarose gels 2,5 25.00 Week 11
Continuous Assessment Completion of an assignment/essay/mid-term examination focused on lecture material and related other recommended reading 1,2,3,4 25.00 Week 7
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Assessment Date
Formal Exam End-of-Semester Final Examination 1,2,3,4 50.00 End-of-Semester

TU Dublin – Tallaght Campus reserves the right to alter the nature and timings of assessment


Module Workload

This module has no Full Time workload.
Workload: Part Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture No Description 3.00 Every Week 3.00
Laboratories No Description 9.00 Every Month 2.25
Assignment Essay/report/mid-term assessment 10.00 Twice per semester 1.33
Total Weekly Learner Workload 6.58
Total Weekly Contact Hours 5.25

Module Resources

Recommended Book Resources
  • Gary Walsh. 2007, Pharmaceutical biotechnology : concepts and applications, John Wiley & Sons Chichester, England ; Hoboken, NJ
  • Helen Kreuzer & Adrianne Massey 2008, Molecular biology and biotechnology : a guide for students
  • Susan R. Barnum 2005, Biotechnology – An introduction, 2nd ed Ed., Thomson
  • G. Walsh 1998, Biopharmaceuticals: Biochemistry and biotechnology, Wiley
  • Bourgaize, Benjamin Cummings 2000, Introduction to biotechnology
  • YC Fung 2001, Introduction to bioengineering, World Sci Publ
  • Sambrook, Fritsch and Maniatis 1989, Molecular Cloning: A Laboratory Manual (Volumes 1, 2 and 3), 2nd ed Ed., Cold Spring Harbour Press
This module does not have any article/paper resources
Other Resources
  • Moodle: All lecture notes, and related learning rercsoues including various current journal articles as appropriate

Module Delivered in

Programme Code Programme Semester Delivery
TA_SPHAR_D Bachelor of Science in Pharmaceutical Technology 5 Group Elective 1